| Product name | Human Myotubularin-related protein 3 (MTMR3) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Myotubularin-related protein 3 (MTMR3) ELISA Kit employs a two-site sandwich ELISA to quantitate MTMR3 in samples. An antibody specific for MTMR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTMR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTMR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTMR3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MTMR3; hCG_2011013; FLJ32333; FYVE-DSP1; KIAA0371; ZFYVE10; FYVE (Fab1 YGLO23 Vsp27 EEA1 domain) dual-specificity protein phosphatase; OTTHUMP00000199667; OTTHUMP00000199668; myotubularin-related protein 3; zi |
| Kit components | • Human Myotubularin-related protein 3 microplate • Human Myotubularin-related protein 3 standard • Human Myotubularin-related protein 3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Myotubularin-related protein 3 (MTMR3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTMR3. No significant cross-reactivity or interference between Human MTMR3 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MTMR3 encodes a member of the myotubularin dual specificity protein phosphatase gene family. Myotubularin-related protein 3 is structurally similar to myotubularin but in addition contains a FYVE domain and an N-terminal PH-GRAM domain. The protein can self-associate and also form heteromers with another myotubularin related protein. The protein binds to phosphoinositide lipids through the PH-GRAM domain, and can hydrolyze phosphatidylinositol(3)-phosphate and phosphatidylinositol(3,5)-biphosphate in vitro. Myotubularin-related protein 3 has been observed to have a perinuclear, possibly membrane-bound, distribution in cells, but it has also been found free in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for MTMR3. |
| Gene ID | 8897 |
| Alternative | MTMR3; hCG_2011013; FLJ32333; FYVE-DSP1; KIAA0371; ZFYVE10; FYVE (Fab1 YGLO23 Vsp27 EEA1 domain) dual-specificity protein phosphatase; OTTHUMP00000199667; OTTHUMP00000199668; myotubularin-related protein 3; zi |
| Accession | Q13615 |
Fig.1. Human Myotubularin-related protein 3 (MTMR3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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