| Product name | Karyopherin α2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Karyopherin α2 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300;WB 1:500-1:2000;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | KPNA2; RCH1; SRP1; Importin subunit alpha-2; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 60kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 (karyopherin subunit alpha 2) protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. |
| Gene ID | 3838 |
| Alternative | KPNA2; RCH1; SRP1; Importin subunit alpha-2; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha |
| Others | Karyopherin α2 Polyclonal Antibody detects endogenous levels of Karyopherin α2 protein. |
| Accession | P52292 |
| Observed Band(KD) | 60 |
Fig. Western blot analysis of Mouse-HEART Mouse-BRAIN using KPNA2 antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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