| Product name | IPKine™ HRP, Mouse Anti-Rabbit IgG HCS |
| Immunogen | Rabbit IgG heavy chain |
| Host | Mouse |
| Reactivity | Rabbit |
| Applications | IP, WB |
| Applications notes | The suggested dilution ratio is: WB: 1:1,000-1:10,000 (1:2000 optimal), IP: 1:1,000-1:10,000 (1:2000 optimal). |
| Clonality | Monoclonal |
| Preparation method | Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally > 95% purity based on SDS-PAGE. |
| Conjugate | HRP |
| Formulation | Liquid solution |
| Features & Benefits | 50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots). |
| Storage buffer | Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. |
| Others | The antibody reacts with kappa heavy chains on rabbit IgG, while it doesn’t react with the light chain of rabbit IgG. It's cross-reaction with human, mouse, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins. |
Fig. Western blot analysis ( lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000, lane 4: 1:8000 ) using IPKine™ HRP, Mouse Anti-Rabbit IgG HCS.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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