Product name | IPKine™ Anti-GST Magnetic IP Kit |
Immunogen | Synthetic Peptide |
Reactivity | Mammals, Bacteria |
Applications | IP |
Applications notes | Anti-Glutathione S-transferase-Tag (GST-Tag) Magnetic Beads are prepared by covalently coupling Anti-GST-Tag Mouse Monoclonal Antibody to crosslinked Magnetic Beads. The Optimized Anti-GST Magnetic Beads have more efficient antigen binding capacity. This kit provides two elution methods, including acid elution and denatured elution. |
Conjugate | Magnetic Beads |
Kit components | Non-Denaturing Lysis Buffer TBS (10×) Anti-GST Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
Features & Benefits | 1.Efficient binding capacity: ≥0.6 mg GST Tag protein/mL Magnetic Beads. • 2.Two elution methods: acid elution and denatured elution. • 3.The kit contains high-quality Anti-GST magnetic beads and all validated immunoprecipitation necessary reagents. • 4.Ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules. |
Storage instructions | Store according to the recommended storage conditions of each component, stable for 12 months. |
Shipping | Gel pack with blue ice. |
Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
Background | The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins. |
Fig. IPKine™ Anti-GST Magnetic IP Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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