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Human Pescadillo homolog (PES1) ELISA Kit

Human Pescadillo homolog (PES1) ELISA Kit

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Specification

Product name Human Pescadillo homolog (PES1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Pescadillo homolog (PES1) ELISA Kit employs a two-site sandwich ELISA to quantitate PES1 in samples. An antibody specific for PES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PES1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PES1; PES; pescadillo homolog 1; containing BRCT domain

Product Properties

Kit components • Human Pescadillo homolog microplate
• Human Pescadillo homolog standard
• Human Pescadillo homolog detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Pescadillo homolog (PES1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PES1. No significant cross-reactivity or interference between Human PES1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PES1 is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.The deduced 588-amino acid PES1 protein is 74% identical to the zebrafish sequence. The authors noted the presence of a highly acidic region at the C terminus and that the protein has an alpha-helix structure. Immunoblot analysis showed that PES1 is expressed as an unmodified 72-kD protein and a SUMO1-modified 92-kD protein. In situ hybridization analysis revealed wide and high expression of Pes1 in embryonic mouse brain and spinal cord. Pes1 expression was upregulated in malignant, late-passage mouse astrocytes.
Gene ID 23481
Alternative PES1; PES; pescadillo homolog 1; containing BRCT domain
Accession O00541

Image & description

Fig.1. Human Pescadillo homolog (PES1) Standard Curve.

Fig.1. Human Pescadillo homolog (PES1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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