| Product name | IPKine™ Anti-DDDDK Magnetic IP Kit |
| Immunogen | Synthetic Peptide |
| Reactivity | Mammals, Bacteria |
| Applications | IP |
| Applications notes | Anti-DDDDK-Tag (Flag-Tag) Magnetic Beads are prepared by covalently coupling Anti-Flag-Tag Mouse Monoclonal Antibody to crosslinked Magnetic Beads. The Optimized Anti-Flag Magnetic Beads have more efficient antigen binding capacity. This kit provides three elution methods, including competitive elution of peptide, acid elution and denatured elution. |
| Conjugate | Magnetic Beads |
| Kit components | Non-Denaturing Lysis Buffer TBS (10×) Anti-Flag Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer 3×Flag Peptide (25×) SDS-PAGE Loading Buffer (5×) |
| Features & Benefits | 1.Efficient binding capacity: ≥0.6 mg Flag Tag protein/mL Magnetic Beads. • 2.Can bind to various forms of tagged proteins: N-/C-. • 3.Three elution methods: competitive elution of peptide, acid elution and denatured elution. • 4.The kit contains high-quality Anti-Flag magnetic beads and all validated immunoprecipitation necessary reagents. • 5.Ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules. |
| Storage instructions | Store according to the recommended storage conditions of each component, stable for 12 months. |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
Fig. IPKine™ Anti-DDDDK Magnetic IP Kit
Author：Liang, Xiao-Ling, et al. Publication name：Journal of Pharmaceutical Analysis IF：
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