| Product name | IPKine™ Anti-DDDDK Magnetic IP Kit |
| Immunogen | Synthetic Peptide |
| Reactivity | Mammals, Bacteria |
| Applications | IP |
| Applications notes | Anti-DDDDK-Tag (Flag-Tag) Magnetic Beads are prepared by covalently coupling Anti-Flag-Tag Mouse Monoclonal Antibody to crosslinked Magnetic Beads. The Optimized Anti-Flag Magnetic Beads have more efficient antigen binding capacity. This kit provides three elution methods, including competitive elution of peptide, acid elution and denatured elution. |
| Conjugate | Magnetic Beads |
| Kit components | Non-Denaturing Lysis Buffer TBS (10×) Anti-Flag Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer 3×Flag Peptide (25×) SDS-PAGE Loading Buffer (5×) |
| Features & Benefits | 1.Efficient binding capacity: ≥0.6 mg Flag Tag protein/mL Magnetic Beads. • 2.Can bind to various forms of tagged proteins: N-/C-. • 3.Three elution methods: competitive elution of peptide, acid elution and denatured elution. • 4.The kit contains high-quality Anti-Flag magnetic beads and all validated immunoprecipitation necessary reagents. • 5.Ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules. |
| Storage instructions | Store according to the recommended storage conditions of each component, stable for 12 months. |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
Fig. IPKine™ Anti-DDDDK Magnetic IP Kit
Author：Liang, Xiao-Ling, et al. Publication name：Journal of Pharmaceutical Analysis IF：
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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