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IL-12A p35 Polyclonal Antibody

IL-12A p35 Polyclonal Antibody

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Specification

Product name IL-12A p35 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human IL-12A p35
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative IL12A; NKSF1; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL12A encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Gene ID 3592
Alternative IL12A; NKSF1; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1
Others IL-12A p35 Polyclonal Antibody detects endogenous levels of IL-12A p35 protein.
Accession P29459
Observed Band(KD) 35

Image & description

Fig.1. Western Blot analysis of MCF7 cells using IL-12A p35 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of MCF7 cells using IL-12A p35 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

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