|Product name||Human Pyrroline-5-carboxylate reductase 3 (PYCRL) ELISA Kit|
|Applications notes||This Human Pyrroline-5-carboxylate reductase 3 (PYCRL) ELISA Kit employs a two-site sandwich ELISA to quantitate PYCRL in samples. An antibody specific for PYCRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYCRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCRL bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Pyrroline-5-carboxylate reductase 3 microplate
• Human Pyrroline-5-carboxylate reductase 3 standard
• Human Pyrroline-5-carboxylate reductase 3 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Pyrroline-5-carboxylate reductase 3 (PYCRL) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYCRL. No significant cross-reactivity or interference between Human PYCRL and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||PYCRL belongs to the pyrroline-5-carboxylate reductase family. Pyrroline-5-carboxylate reductase catalyzes the the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. Pyrroline-5-carboxylate reductase(P5CR) is the enzyme that catalyzes the terminal step in the biosynthesis of proline from glutamate, the NAD(P) dependent oxidation of 1-pyrroline-5-carboxylate into proline. Pyrroline-5-carboxylate reductase, which converts pyrroline-5-carboxylate to proline, has been identified in human erythrocytes. The level of pyrroline-5-carboxylate reductase activity in these cells is comparable to the activity levels of major erythrocyte enzymes. The physiologic function of the enzyme in erythrocytes cannot be related to its function in other tissues.|
Fig.1. Human Pyrroline-5-carboxylate reductase 3 (PYCRL) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.