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IF2M Polyclonal Antibody

IF2M Polyclonal Antibody

Views(13) Publications(0) Catalog no(ABP58871)
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Specification

Product name IF2M Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human IF2M protein at amino acid sequence of 450-530
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Translation initiation factor IF-2, mitochondrial; IF-2Mt

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 79kD
Storage buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MTIF2 (Mitochondrial Translational Initiation Factor 2) is a Protein Coding gene. Among its related pathways are Mitochondrial translation and Organelle biogenesis and maintenance. During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins.
Gene ID 4528
Alternative Translation initiation factor IF-2, mitochondrial; IF-2Mt
Others The antibody detects endogenous levels of IF2M protein
Accession P46199
Observed Band(KD) 79

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