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Human Vitamin K-dependent protein Z (PROZ) ELISA Kit

Human Vitamin K-dependent protein Z (PROZ) ELISA Kit

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Specification

Product name Human Vitamin K-dependent protein Z (PROZ) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Vitamin K-dependent protein Z (PROZ) ELISA Kit employs a two-site sandwich ELISA to quantitate PROZ in samples. An antibody specific for PROZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PROZ; PZ;protein Z; vitamin K-dependent plasma glycoprotein

Product Properties

Kit components • Human Vitamin K-dependent protein Z microplate
• Human Vitamin K-dependent protein Z standard
• Human Vitamin K-dependent protein Z detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Vitamin K-dependent protein Z (PROZ) ELISA Kit has high sensitivity and excellent specificity for detection of Human PROZ. No significant cross-reactivity or interference between Human PROZ and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protein Z is a member of the coagulation cascade, the group of blood proteins that leads to the formation of blood clots. It is vitamin K-dependent, and its functionality is therefore impaired in warfarin therapy. It is a glycoprotein. Structural analysis of protein Z will allow better understanding of its function. From a Ramachandran plot, the secondary structure of Protein Z was determined. The Ramachandran plot utilizes mathematical equations to determine the possible angles of the amino acids within the primary sequence of Protein Z. The possible angles results in a plot of possible secondary structures. The Ramachandran plot for protein Z indicates it will form alpha helices. The final structure, all aphla domain, was determined by x-ray diffraction. It consists of chain A and B, which are both helix-loop-helix motifs.
Gene ID 8858
Alternative PROZ; PZ;protein Z; vitamin K-dependent plasma glycoprotein
Accession P22891

Image & description

Fig.1. Human Vitamin K-dependent protein Z (PROZ) Standard Curve.

Fig.1. Human Vitamin K-dependent protein Z (PROZ) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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