| Product name | Recombinant SARS-CoV-2 Spike RBD Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from SARS-CoV-2 (COVID-19) Spike RBD protein (YP_009724390.1, Arg 319 - Phe 541) was expressed with a polyhistidine tag at the C-terminus. |
| Activity | Immobilized SARS-CoV-2 Spike RBD protein at 1.25 μg/mL (100μL/well) can bind Human Ace-2, the EC50 of human Ace-2 is 54 ng/mL. |
| Protein length | The recombinant SARS-CoV-2 (COVID-19) Spike RBD protein consists of 229 amino acids and predicts a molecular mass of 26.5 kD.The apparent molecular mass of the human CD33 is approximately 32-35 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method | HEK 293 Cells |
| Purity | >95% as determined by SDS-PAGE |
| Alternative | SARS-CoV-2-Spike-RBD，RBD，SARS-CoV-2 Spike RBD，SARS-CoV-2 SP RBD，2019-nCoV SARS-CoV-2 Spike Protein (RBD) |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Features & Benefits | Endotoxin: < 1 EU per µg of the protein as determined by the LAL method. |
| Molecular weight | 26.5kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized SARS-CoV-2 (COVID-19) S protein RBD protein not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Recombinant SARS-CoV-2 Spike RBD Protein, His tag (Animal-Free)should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.121% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | COVID-19 has become pandemic with higher morbidity and mortality rates after its start from Wuhan city of China. The infection by RNA virus, also known as SARS-CoV-2 or 2019-nCoV, from β class of corona viruses has been found to be responsible for COVID-19. Structural analysis and evidences have been indicated that interaction between a segment of receptor binding domain (RBD) from S protein of virus and human angiotensin-converting enzyme 2 (hACE2) is essential for cellular entry of virus. Inhibition of RBD-hACE2 interaction by different molecular scaffolds can be used as a preferred strategy for control of SARS-CoV-2 infection. |
| Gene ID | 43740568 |
| Alternative | SARS-CoV-2-Spike-RBD，RBD，SARS-CoV-2 Spike RBD，SARS-CoV-2 SP RBD，2019-nCoV SARS-CoV-2 Spike Protein (RBD) |
| Accession | P0DTC2 |
Fig.SDS-PAGE analysis of Recombinant SARS-CoV-2 Spike RBD Protein, His tag
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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