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Human Vasorin (VASN) ELISA Kit

Human Vasorin (VASN) ELISA Kit

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Product name Human Vasorin (VASN) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Vasorin (VASN) ELISA Kit employs a two-site sandwich ELISA to quantitate VASN in samples. An antibody specific for VASN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVASN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative VASN; SLITL2; slit-like 2

Product Properties

Kit components • Human Vasorin microplate
• Human Vasorin standard
• Human Vasorin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Vasorin (VASN) ELISA Kit has high sensitivity and excellent specificity for detection of Human VASN. No significant cross-reactivity or interference between Human VASN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 673-amino acid protein contains a putative hydrophobic signal sequence, 10 tandem arrays of a leucine-rich repeat, an epidermal growth factor-like domain, a fibronectin type III-like domain, and a short intracellular region. In situ hybridization detected mouse vasorin in the tunica media of the proximal ascending aorta, descending thoracic aorta, abdominal aorta, and coronary arteries. In mouse kidney, vasorin was expressed in interstitial cells. expression of vasorin in aortas increased gradually in parallel with the differentiation of vascular smooth muscle cells beginning at embryonic day 10.5. Vasorin expressed by transfected Chinese hamster ovary cells showed an apparent molecular mass of 110 kD, which shifted following N-glycosidase treatment. Vasorin was expressed on the cell surface and was secreted into the culture medium.
Gene ID 114990
Alternative VASN; SLITL2; slit-like 2
Accession Q6EMK4

Image & description

Fig.1. Human Vasorin (VASN) Standard Curve.

Fig.1. Human Vasorin (VASN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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