| Product name | Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM3 in samples. An antibody specific for MCM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MCM3; HCC5; MGC1157; P1-MCM3; P1.h; RLFB; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; MCM3 minichromosome maintenance deficient 3; cervical cancer proto-oncogene 5; hRl |
| Kit components | • Human DNA replication licensing factor MCM3 microplate • Human DNA replication licensing factor MCM3 standard • Human DNA replication licensing factor MCM3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human DNA replication licensing factor MCM3 (MCM3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MCM3. No significant cross-reactivity or interference between Human MCM3 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. |
| Gene ID | 4172 |
| Alternative | MCM3; HCC5; MGC1157; P1-MCM3; P1.h; RLFB; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; MCM3 minichromosome maintenance deficient 3; cervical cancer proto-oncogene 5; hRl |
| Accession | P25205 |
Fig.1. Human DNA replication licensing factor MCM3 (MCM3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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