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Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit

Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit

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Specification

Product name Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPA in samples. An antibody specific for SIRPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SIRPA; BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS-1; SHPS1; SIRP; SIRP-ALPHA-1; SIRPalpha; SIRPalpha2; SHP substrate-1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; macrophage fu

Product Properties

Kit components • Human Tyrosine-protein phosphatase non-receptor type substrate 1 microplate
• Human Tyrosine-protein phosphatase non-receptor type substrate 1 standard
• Human Tyrosine-protein phosphatase non-receptor type substrate 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRPA. No significant cross-reactivity or interference between Human SIRPA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Signal-regulatory protein alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family.
Gene ID 140885
Alternative SIRPA; BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS-1; SHPS1; SIRP; SIRP-ALPHA-1; SIRPalpha; SIRPalpha2; SHP substrate-1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; macrophage fu
Accession P78324

Image & description

Fig.1. Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) Standard Curve.

Fig.1. Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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