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Talin-1 Polyclonal Antibody

Talin-1 Polyclonal Antibody

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Specification

Product name Talin-1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Talin-1
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative TLN1; KIAA1027; TLN; Talin-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TLN1 encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The talin 1 plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Gene ID 7094
Alternative TLN1; KIAA1027; TLN; Talin-1
Others Talin-1 Polyclonal Antibody detects endogenous levels of Talin-1 protein.
Accession Q9Y490
Observed Band(KD) 269

Image & description

Fig.1. Western Blot analysis of K562 cells using Talin-1 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of K562 cells using Talin-1 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.

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