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Human Tryptophan hydroxylase (TPH) ELISA Kit

Human Tryptophan hydroxylase (TPH) ELISA Kit

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Specification

Product name Human Tryptophan hydroxylase (TPH) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tryptophan hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1 in samples. An antibody specific for TPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TPH1; MGC119994; TPRH; TRPH; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)

Product Properties

Kit components • Human Tryptophan hydroxylase microplate
• Human Tryptophan hydroxylase standard
• Human Tryptophan hydroxylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tryptophan hydroxylase (TPH) ELISA Kit has high sensitivity and excellent specificity for detection of Human TPH1. No significant cross-reactivity or interference between Human TPH1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TPH1 encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others. Tryptophan hydroxylase is important for the brain. However, Tph1 is not detected in the brain. Nevertheless the effect of variations in the TPH1 gene on brain-related variables, such as personality traits and neuropsychiatric disorders, has been studied. One human mutant of TPH1, A218C found in intron 7, is highly associated with schizophrenia.
Gene ID 7166
Alternative TPH1; MGC119994; TPRH; TRPH; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)
Accession P17752

Image & description

Fig.1. Human Tryptophan hydroxylase (TPH) Standard Curve.

Fig.1. Human Tryptophan hydroxylase (TPH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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