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Human Troponin I, fast skeletal muscle (TNNI2) ELISA Kit

Human Troponin I, fast skeletal muscle (TNNI2) ELISA Kit

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Specification

Product name Human Troponin I, fast skeletal muscle (TNNI2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Troponin I, fast skeletal muscle (TNNI2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNI2 in samples. An antibody specific for TNNI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNNI2; AMCD2B; DA2B; FSSV; fsTnI; OTTHUMP00000014141; OTTHUMP00000014142; fast-twitch skeletal muscle troponin I; troponin I fast twitch 2; troponin I; fast-twitch skeletal muscle isoform; troponin I; skeletal;

Product Properties

Kit components • Human Troponin I, fast skeletal muscle microplate
• Human Troponin I, fast skeletal muscle standard
• Human Troponin I, fast skeletal muscle detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Troponin I, fast skeletal muscle (TNNI2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNNI2. No significant cross-reactivity or interference between Human TNNI2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNNI2 is a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma.
Gene ID 7136
Alternative TNNI2; AMCD2B; DA2B; FSSV; fsTnI; OTTHUMP00000014141; OTTHUMP00000014142; fast-twitch skeletal muscle troponin I; troponin I fast twitch 2; troponin I; fast-twitch skeletal muscle isoform; troponin I; skeletal;
Accession P48788

Image & description

Fig.1. Human Troponin I, fast skeletal muscle (TNNI2) Standard Curve.

Fig.1. Human Troponin I, fast skeletal muscle (TNNI2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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