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Human Transforming growth factor β receptor 2 (TGF-βR2) ELISA Kit

Human Transforming growth factor β receptor 2 (TGF-βR2) ELISA Kit

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Specification

Product name Human Transforming growth factor β receptor 2 (TGF-βR2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transforming growth factor β receptor 2 (TGF-βR2) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFBR2 in samples. An antibody specific for TGFBR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFBR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TGFBR2; AAT3; FAA3; LDS1B; LDS2B; MFS2; RIIC; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta receptor typeB; TGF-beta type receptor; transforming growth factor beta receptor typeC; transforming growth factor; b

Product Properties

Kit components • Human Transforming growth factor β receptor 2 microplate
• Human Transforming growth factor β receptor 2 standard
• Human Transforming growth factor β receptor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transforming growth factor β receptor 2 (TGF-βR2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TGFBR2. No significant cross-reactivity or interference between Human TGFBR2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Transforming growth factor β receptor 2 (TGF-βR2) is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. TGF-βR2 is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGF-βR2 have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.
Gene ID 7048
Alternative TGFBR2; AAT3; FAA3; LDS1B; LDS2B; MFS2; RIIC; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta receptor typeB; TGF-beta type receptor; transforming growth factor beta receptor typeC; transforming growth factor; b
Accession P37173

Image & description

Fig.1. Human Transforming growth factor β receptor 2 (TGF-βR2) Standard Curve.

Fig.1. Human Transforming growth factor β receptor 2 (TGF-βR2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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