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Human Tensin-1 (TNS1) ELISA Kit

Human Tensin-1 (TNS1) ELISA Kit

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Specification

Product name Human Tensin-1 (TNS1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tensin-1 (TNS1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS1 in samples. An antibody specific for TNS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNS1; MSTP091; DKFZp586K0617; MGC88584; MST091; MST122; MST127; MSTP122; MSTP127; MXRA6; TNS; Matrix-remodelling-associated protein 6; matrix-remodelling associated 6; tensin

Product Properties

Kit components • Human Tensin-1 microplate
• Human Tensin-1 standard
• Human Tensin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tensin-1 (TNS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNS1. No significant cross-reactivity or interference between Human TNS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tensin-1 is a protein localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. A second transcript from this gene has been described, but its full length nature has not been determined. This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and Northern Blot. May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton.
Gene ID 7145
Alternative TNS1; MSTP091; DKFZp586K0617; MGC88584; MST091; MST122; MST127; MSTP122; MSTP127; MXRA6; TNS; Matrix-remodelling-associated protein 6; matrix-remodelling associated 6; tensin
Accession Q9HBL0

Image & description

Fig.1. Human Tensin-1 (TNS1) Standard Curve.

Fig.1. Human Tensin-1 (TNS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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