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Human Myosin light chain kinase (MLCK) ELISA Kit

Human Myosin light chain kinase (MLCK) ELISA Kit

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Specification

Product name Human Myosin light chain kinase (MLCK) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Myosin light chain kinase (MLCK) ELISA Kit employs a two-site sandwich ELISA to quantitate MYLK in samples. An antibody specific for MYLK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYLK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYLK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYLK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYLK; DKFZp686I10125; FLJ12216; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK; OTTHUMP00000180642; myosin; light polypeptide kinase; smooth muscle myosin light chain kinase

Product Properties

Kit components • Human Myosin light chain kinase microplate
• Human Myosin light chain kinase standard
• Human Myosin light chain kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Myosin light chain kinase (MLCK) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYLK. No significant cross-reactivity or interference between Human MYLK and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MLCK, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. MLCK encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
Gene ID 4638
Alternative MYLK; DKFZp686I10125; FLJ12216; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK; OTTHUMP00000180642; myosin; light polypeptide kinase; smooth muscle myosin light chain kinase
Accession Q15746

Image & description

Fig.1. Human Myosin light chain kinase (MLCK) Standard Curve.

Fig.1. Human Myosin light chain kinase (MLCK) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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