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Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit

Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit

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Specification

Product name Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R2 in samples. An antibody specific for TAS1R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS1R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS1R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAS1R2; GPR71; T1R2; TR2; G protein-coupled receptor 71

Product Properties

Kit components • Human Taste receptor type 1 member 2 microplate
• Human Taste receptor type 1 member 2 standard
• Human Taste receptor type 1 member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Taste receptor type 1 member 2 (TAS1R2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAS1R2. No significant cross-reactivity or interference between Human TAS1R2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Taste receptor type 1 member 2 (TAS1R2) is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R2+3. TAS1R2 is formed as a dimer of the TAS1R2 and TAS1R3 proteins. Moreover, the TAS1R2 protein is not functional without formation of the 2+3 heterodimer. Another interesting quality of the TAS1R2 and TAS1R1 genes is their spontaneous activity in the absence of the extracellular domains and binding ligands. This may mean that the extracellular domain regulates function of the receptor by preventing spontaneous action as well as binding to activating ligands such as sucrose.
Gene ID 80834
Alternative TAS1R2; GPR71; T1R2; TR2; G protein-coupled receptor 71
Accession Q8TE23

Image & description

Fig.1. Human Taste receptor type 1 member 2 (TAS1R2) Standard Curve.

Fig.1. Human Taste receptor type 1 member 2 (TAS1R2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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