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Human Suppressor of tumorigenicity 14 protein (ST14) ELISA Kit

Human Suppressor of tumorigenicity 14 protein (ST14) ELISA Kit

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Specification

Product name Human Suppressor of tumorigenicity 14 protein (ST14) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Suppressor of tumorigenicity 14 protein (ST14) ELISA Kit employs a two-site sandwich ELISA to quantitate ST14 in samples. An antibody specific for ST14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyST14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ST14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ST14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ST14; HAI; MT-SP1; MTSP1; PRSS14; SNC19; TADG15; matriptase; membrane-type serine protease 1; prostamin; serine protease 14; serine protease TADG-15; suppression of tumorigenicity 14 (colon carcinoma; matripta

Product Properties

Kit components • Human Suppressor of tumorigenicity 14 protein microplate
• Human Suppressor of tumorigenicity 14 protein standard
• Human Suppressor of tumorigenicity 14 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Suppressor of tumorigenicity 14 protein (ST14) ELISA Kit has high sensitivity and excellent specificity for detection of Human ST14. No significant cross-reactivity or interference between Human ST14 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ST14 is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. ST14 lacks a signal sequence and is likely to be a membrane-associated serine protease.Expression was also observed in a colorectal adenocarcinoma cell line, but not in several other cell lines tested.
Gene ID 6768
Alternative ST14; HAI; MT-SP1; MTSP1; PRSS14; SNC19; TADG15; matriptase; membrane-type serine protease 1; prostamin; serine protease 14; serine protease TADG-15; suppression of tumorigenicity 14 (colon carcinoma; matripta
Accession Q9Y5Y6

Image & description

Fig.1. Human Suppressor of tumorigenicity 14 protein (ST14) Standard Curve.

Fig.1. Human Suppressor of tumorigenicity 14 protein (ST14) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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