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Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit

Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit

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Specification

Product name Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT6 in samples. An antibody specific for STAT6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative STAT6; D12S1644; IL-4-STAT; STAT6B; STAT6C; STAT; interleukin4-induced; signal transducer and activator of transcription 6; transcription factor IL-4 STAT

Product Properties

Kit components • Human Signal transducer and activator of transcription 6 microplate
• Human Signal transducer and activator of transcription 6 standard
• Human Signal transducer and activator of transcription 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Signal transducer and activator of transcription 6 (STAT6) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAT6. No significant cross-reactivity or interference between Human STAT6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.
Gene ID 6778
Alternative STAT6; D12S1644; IL-4-STAT; STAT6B; STAT6C; STAT; interleukin4-induced; signal transducer and activator of transcription 6; transcription factor IL-4 STAT
Accession P42226

Image & description

Fig.1. Human Signal transducer and activator of transcription 6 (STAT6) Standard Curve.

Fig.1. Human Signal transducer and activator of transcription 6 (STAT6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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