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Human Sodium-dependent serotonin transporter (SLC6A4) ELISA Kit

Human Sodium-dependent serotonin transporter (SLC6A4) ELISA Kit

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Specification

Product name Human Sodium-dependent serotonin transporter (SLC6A4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Sodium-dependent serotonin transporter (SLC6A4) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A4 in samples. An antibody specific for SLC6A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC6A4; 5-HTT; 5-HTTLPR; 5HTT; HTT; OCD1; SERT; hSERT; 5-hydroxytryptamine transporter; 5HT transporter; Na+/Cl- dependent serotonin transporter; OTTHUMP00000163633; sodium-dependent serotonin transporter; solut

Product Properties

Kit components • Human Sodium-dependent serotonin transporter microplate
• Human Sodium-dependent serotonin transporter standard
• Human Sodium-dependent serotonin transporter detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Sodium-dependent serotonin transporter (SLC6A4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A4. No significant cross-reactivity or interference between Human SLC6A4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The serotonin transporter (SERT) is a monoamine transporter protein.This protein is an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamine, cocaine, and MDMA, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
Gene ID 6532
Alternative SLC6A4; 5-HTT; 5-HTTLPR; 5HTT; HTT; OCD1; SERT; hSERT; 5-hydroxytryptamine transporter; 5HT transporter; Na+/Cl- dependent serotonin transporter; OTTHUMP00000163633; sodium-dependent serotonin transporter; solut
Accession P31645

Image & description

Fig.1. Human Sodium-dependent serotonin transporter (SLC6A4) Standard Curve.

Fig.1. Human Sodium-dependent serotonin transporter (SLC6A4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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