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Human Serpin B5 (SERPINB5) ELISA Kit

Human Serpin B5 (SERPINB5) ELISA Kit

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Specification

Product name Human Serpin B5 (SERPINB5) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Serpin B5 (SERPINB5) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB5 in samples. An antibody specific for SERPINB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINB5; PI5; maspin; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 5

Product Properties

Kit components • Human Serpin B5 microplate
• Human Serpin B5 standard
• Human Serpin B5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Serpin B5 (SERPINB5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINB5. No significant cross-reactivity or interference between Human SERPINB5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Maspin is a serpin and tumor suppressor gene.It has been associated with prostate cancer and breast cancer.It has also been associated with tumors of the pancreas.It was identified in 1994.In normal cells expressing SERPINB5, the SERPINB5 promoter is unmethylated and the promoter region has acetylated histones and an accessible chromatin structure. In contrast, normal cells that do not express SERPINB5 have a completely methylated SERPINB5 promoter with hypoacetylated histones, an inaccessible chromatin structure, and a transcriptional repression that is relieved by inhibition of DNA methylation. These findings indicated that cytosine methylation is important in the establishment and maintenance of cell type-restricted gene expression.
Gene ID 5268
Alternative SERPINB5; PI5; maspin; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 5
Accession P36952

Image & description

Fig.1. Human Serpin B5 (SERPINB5) Standard Curve.

Fig.1. Human Serpin B5 (SERPINB5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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