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Human Semenogelin-2 (SEMG2) ELISA Kit

Human Semenogelin-2 (SEMG2) ELISA Kit

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Specification

Product name Human Semenogelin-2 (SEMG2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Semenogelin-2 (SEMG2) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMG2 in samples. An antibody specific for SEMG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SEMG2; SGII; Semenogelin 2

Product Properties

Kit components • Human Semenogelin-2 microplate
• Human Semenogelin-2 standard
• Human Semenogelin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Semenogelin-2 (SEMG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMG2. No significant cross-reactivity or interference between Human SEMG2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SEMG2 is involved in the formation of a gel matrix that encases ejaculated spermatozoa. Proteolysis by the prostate-specific antigen (PSA) breaks down the gel matrix and allows the spermatozoa to move more freely. The encoded protein is found in lesser abundance than a similar semenogelin protein. The genes encoding these two semenogelin proteins are found in a cluster on chromosome 20. The transcription units are composed of 3 exons, of which the first encodes the signal peptide, the second encodes secreted protein, and the third contains solely 3-prime, noncoding nucleotides. The nucleotide sequences exhibited a similarity of close to 90% in the exons and greater than 80% in the introns and flanking nucleotides.
Gene ID 6407
Alternative SEMG2; SGII; Semenogelin 2
Accession Q02383

Image & description

Fig.1. Human Semenogelin-2 (SEMG2) Standard Curve.

Fig.1. Human Semenogelin-2 (SEMG2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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