| Product name | Human Phosphatidylethanolamine-binding protein 1 (PEBP1) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Phosphatidylethanolamine-binding protein 1 (PEBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PEBP1 in samples. An antibody specific for PEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PEBP1; HCNP; PBP; PEBP; RKIP; Raf kinase inhibitory protein; hippocampal cholinergic neurostimulating peptide; prostatic binding protein |
| Kit components | • Human Phosphatidylethanolamine-binding protein 1 microplate • Human Phosphatidylethanolamine-binding protein 1 standard • Human Phosphatidylethanolamine-binding protein 1 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Phosphatidylethanolamine-binding protein 1 (PEBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PEBP1. No significant cross-reactivity or interference between Human PEBP1 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | RKIP cDNA encodes a protein of 186 amino acids, the sequence of which is 95% identical to the bovine 21- to 23-kD protein. The rat protein shows 85.5% identity with the human protein. highly expressed in the brain and associated with cytosolic proteins and small GTP-binding proteins. Multiple tissue Northern blots revealed the presence of a single mRNA in the different tissues of each species; a single band of 1.8 kb was identified in human, 1.45 kb in mouse, and 1.2 kb in rat tissues. The mRNA is particularly highly expressed in rat and mouse testis, where the level was 30 times higher than that in the brain. PBP is expressed strongly in the cell bodies of oligodendrocytes but only weakly elsewhere. Schwann cells and spinal nerve roots showed intense cytoplasmic PBP immunoreactivity. |
| Gene ID | 5037 |
| Alternative | PEBP1; HCNP; PBP; PEBP; RKIP; Raf kinase inhibitory protein; hippocampal cholinergic neurostimulating peptide; prostatic binding protein |
| Accession | P30086 |
Fig.1. Human Phosphatidylethanolamine-binding protein 1 (PEBP1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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