| Product name | Human Retrotransposon-derived protein PEG10 (PEG10) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Retrotransposon-derived protein PEG10 (PEG10) ELISA Kit employs a two-site sandwich ELISA to quantitate PEG10 in samples. An antibody specific for PEG10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEG10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEG10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEG10 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PEG10; Edr; HB-1; KIAA1051; MEF3L; Mar2; Mart2; RGAG3; MEF3 like 1; embryonal carcinoma differentiation regulated; retrotransposon gag domain containing 3 |
| Kit components | • Human Retrotransposon-derived protein PEG10 microplate • Human Retrotransposon-derived protein PEG10 standard • Human Retrotransposon-derived protein PEG10 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Retrotransposon-derived protein PEG10 (PEG10) ELISA Kit has high sensitivity and excellent specificity for detection of Human PEG10. No significant cross-reactivity or interference between Human PEG10 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frameshifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas PEG10 is a paternally expressed imprinted gene that is expressed in adult and embryonic tissues.Most notable expression occurs in the placenta. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC). This gene has been reported to play a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. In preeclampsia placental tissue, PEG10 has been shown to be downregulated[8] and upregulated[9] implicating it as a possible causal role in the occurrence of preeclampsia. |
| Gene ID | 23089 |
| Alternative | PEG10; Edr; HB-1; KIAA1051; MEF3L; Mar2; Mart2; RGAG3; MEF3 like 1; embryonal carcinoma differentiation regulated; retrotransposon gag domain containing 3 |
| Accession | Q86TG7 |
Fig.1. Human Retrotransposon-derived protein PEG10 (PEG10) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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