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Human Homeobox protein TGIF2LX (TGIF2LX) ELISA Kit

Human Homeobox protein TGIF2LX (TGIF2LX) ELISA Kit

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Specification

Product name Human Homeobox protein TGIF2LX (TGIF2LX) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Homeobox protein TGIF2LX (TGIF2LX) ELISA Kit employs a two-site sandwich ELISA to quantitate TGIF2LX in samples. An antibody specific for TGIF2LX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGIF2LX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGIF2LX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGIF2LX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TGIF2LX; MGC34726; TGIFLX; TGF(beta)-induced transcription factor 2-like; TGFB-induced factor 2-like; X-linked

Product Properties

Kit components • Human Homeobox protein TGIF2LX microplate
• Human Homeobox protein TGIF2LX standard
• Human Homeobox protein TGIF2LX detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Homeobox protein TGIF2LX (TGIF2LX) ELISA Kit has high sensitivity and excellent specificity for detection of Human TGIF2LX. No significant cross-reactivity or interference between Human TGIF2LX and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Homeobox protein TGIF2LX (TGIF2LX) is a member of the TALE/TGIF homeobox family of transcription factors. Testis-specific expression suggests that TGIF2LX may play a role in spermatogenesis. A homolog of this gene lies within the male specific region of chromosome Y, in a block of sequence that is thought to be the result of a large X-to-Y transposition.
Gene ID 90316
Alternative TGIF2LX; MGC34726; TGIFLX; TGF(beta)-induced transcription factor 2-like; TGFB-induced factor 2-like; X-linked
Accession Q8IUE1

Image & description

Fig.1. Human Homeobox protein TGIF2LX (TGIF2LX) Standard Curve.

Fig.1. Human Homeobox protein TGIF2LX (TGIF2LX) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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