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Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit

Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit

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Specification

Product name Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA4 in samples. An antibody specific for ABCA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ABCA4; ABC10; ABCR; ARMD2; CORD3; DKFZp781N1972; FFM; FLJ17534; RMP; RP19; STGD; STGD1; ATP binding cassette transporter; ATP-binding cassette; sub-family A member 4; ATP-binding transporter; retina-specific

Product Properties

Kit components • Human Retinal-specific ATP-binding cassette transporter microplate
• Human Retinal-specific ATP-binding cassette transporter standard
• Human Retinal-specific ATP-binding cassette transporter detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Retinal-specific ATP-binding cassette transporter (ABCA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCA4. No significant cross-reactivity or interference between Human ABCA4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ABCA4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies. This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essental molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macular degeneration.
Gene ID 24
Alternative ABCA4; ABC10; ABCR; ARMD2; CORD3; DKFZp781N1972; FFM; FLJ17534; RMP; RP19; STGD; STGD1; ATP binding cassette transporter; ATP-binding cassette; sub-family A member 4; ATP-binding transporter; retina-specific
Accession P78363

Image & description

Fig.1. Human Retinal-specific ATP-binding cassette transporter (ABCA4) Standard Curve.

Fig.1. Human Retinal-specific ATP-binding cassette transporter (ABCA4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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