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Human Renin (REN) ELISA Kit

Human Renin (REN) ELISA Kit

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Specification

Product name Human Renin (REN) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Renin (REN) ELISA Kit employs a two-site sandwich ELISA to quantitate REN in samples. An antibody specific for REN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative REN; FLJ10761; HNFJ2; angiotensin-forming enzyme; angiotensinogenase; renin precursor; renal

Product Properties

Kit components • Human Renin microplate
• Human Renin standard
• Human Renin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Renin (REN) ELISA Kit has high sensitivity and excellent specificity for detection of Human REN. No significant cross-reactivity or interference between Human REN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney.Renin differs from the other members of this class by having a pH optimum near neutral pH region with native substrates instead of 2.0 to 3.4 This more neutral pH optimum allows it to be functional in the plasma. Renin also has very high selectivity for substrate due to quite long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin.
Gene ID 5972
Alternative REN; FLJ10761; HNFJ2; angiotensin-forming enzyme; angiotensinogenase; renin precursor; renal
Accession P00797

Image & description

Fig.1. Human Renin (REN) Standard Curve.

Fig.1. Human Renin (REN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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