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Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) ELISA Kit

Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) ELISA Kit

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Specification

Product name Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRJ in samples. An antibody specific for PTPRJ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRJ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRJ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRJ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTPRJ; CD148; DEP1; HPTPeta; R-PTP-ETA; SCC1; CD148 antigen; human density enhanced phosphatase-1; protein tyrosine phosphatase; receptor type; J polypeptide; receptor-type tyrosine-protein phosphatase eta; su

Product Properties

Kit components • Human Receptor-type tyrosine-protein phosphatase eta microplate
• Human Receptor-type tyrosine-protein phosphatase eta standard
• Human Receptor-type tyrosine-protein phosphatase eta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPRJ. No significant cross-reactivity or interference between Human PTPRJ and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This PTP is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). This PTP was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction.
Gene ID 5795
Alternative PTPRJ; CD148; DEP1; HPTPeta; R-PTP-ETA; SCC1; CD148 antigen; human density enhanced phosphatase-1; protein tyrosine phosphatase; receptor type; J polypeptide; receptor-type tyrosine-protein phosphatase eta; su
Accession Q12913

Image & description

Fig.1. Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) Standard Curve.

Fig.1. Human Receptor-type tyrosine-protein phosphatase eta (PTPRJ) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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