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Human Protein kinase C alpha type (PRKCA) ELISA Kit

Human Protein kinase C alpha type (PRKCA) ELISA Kit

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Specification

Product name Human Protein kinase C alpha type (PRKCA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein kinase C alpha type (PRKCA) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKCA in samples. An antibody specific for PRKCA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRKCA; AAG6; MGC129900; MGC129901; PKC-alpha; PKCA; PRKACA; aging-associated gene 6; protein kinase C alpha type

Product Properties

Kit components • Human Protein kinase C alpha type microplate
• Human Protein kinase C alpha type standard
• Human Protein kinase C alpha type detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein kinase C alpha type (PRKCA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKCA. No significant cross-reactivity or interference between Human PRKCA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PRKCA, refers to both a human gene and the protein that is encoded by it. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control.
Gene ID 5578
Alternative PRKCA; AAG6; MGC129900; MGC129901; PKC-alpha; PKCA; PRKACA; aging-associated gene 6; protein kinase C alpha type
Accession P17252

Image & description

Fig.1. Human Protein kinase C alpha type (PRKCA) Standard Curve.

Fig.1. Human Protein kinase C alpha type (PRKCA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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