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Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit

Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit

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Specification

Product name Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit employs a two-site sandwich ELISA to quantitate SCXA in samples. An antibody specific for SCXA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCXA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCXA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCXA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SCXA; SCX; bHLHa41; class bHLH protein scleraxis; scleraxis homolog A

Product Properties

Kit components • Human Basic helix-loop-helix transcription factor scleraxis microplate
• Human Basic helix-loop-helix transcription factor scleraxis standard
• Human Basic helix-loop-helix transcription factor scleraxis detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCXA. No significant cross-reactivity or interference between Human SCXA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background By real-time PCR, Schulze-Tanzil et al. (2004) found that expression of SCX, a tendon-specific marker, increased in human tenocytes during their entire time in high-density cultures.By in situ hybridization, Brent et al. (2003) found that Scx was expressed in axial and ventrolateral body wall tendons during chicken embryonic development. It was also expressed in a population of tendon progenitors in developing somites. Brent et al. (2003) determined that FGF signaling in the myotome was both necessary and sufficient for induction of Scx expression in developing chicken somites. Overexpression of Pax1 inhibited Scx expression in somites.Hartz (2004) mapped the SCX gene to chromosome 8q24.3 based on an alignment of the SCX sequence with the genomic sequence.
Gene ID 642658
Alternative SCXA; SCX; bHLHa41; class bHLH protein scleraxis; scleraxis homolog A
Accession Q7RTU7

Image & description

Fig.1. Human Basic helix-loop-helix transcription factor scleraxis (SCXA) Standard Curve.

Fig.1. Human Basic helix-loop-helix transcription factor scleraxis (SCXA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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