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Human Kunitz-type protease inhibitor 2 (SPINT2) ELISA Kit

Human Kunitz-type protease inhibitor 2 (SPINT2) ELISA Kit

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Specification

Product name Human Kunitz-type protease inhibitor 2 (SPINT2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Kunitz-type protease inhibitor 2 (SPINT2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPINT2 in samples. An antibody specific for SPINT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPINT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPINT2; DIAR3; FLJ45571; HAI-2; HAI2; Kop; PB; hepatocyte growth factor activator inhibitor type 2; placental bikunin; serine protease inhibitor; Kunitz type; 2

Product Properties

Kit components • Human Kunitz-type protease inhibitor 2 microplate
• Human Kunitz-type protease inhibitor 2 standard
• Human Kunitz-type protease inhibitor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Kunitz-type protease inhibitor 2 (SPINT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPINT2. No significant cross-reactivity or interference between Human SPINT2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SPINT2 encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. Kunitz-type protease inhibitor 2 inhibits HGF activator which prevents the formation of active hepatocyte growth factor. SPINT2 is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for SPINT2.
Gene ID 10653
Alternative SPINT2; DIAR3; FLJ45571; HAI-2; HAI2; Kop; PB; hepatocyte growth factor activator inhibitor type 2; placental bikunin; serine protease inhibitor; Kunitz type; 2
Accession O43291

Image & description

Fig.1. Human Kunitz-type protease inhibitor 2 (SPINT2) Standard Curve.

Fig.1. Human Kunitz-type protease inhibitor 2 (SPINT2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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