Login Register
English
0

Cart

$ 0

Human Prostaglandin reductase 2 (PTGR2) ELISA Kit

Human Prostaglandin reductase 2 (PTGR2) ELISA Kit

Views(133) Publications(0) Catalog no(KTE61067)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Prostaglandin reductase 2 (PTGR2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Prostaglandin reductase 2 (PTGR2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGR2 in samples. An antibody specific for PTGR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTGR2; DKFZp686P10120; FLJ39091; FLJ99229; PGR2; ZADH1; 15-oxoprostaglandin-delta13-reductase; zinc binding alcohol dehydrogenase; domain containing 1

Product Properties

Kit components • Human Prostaglandin reductase 2 microplate
• Human Prostaglandin reductase 2 standard
• Human Prostaglandin reductase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Prostaglandin reductase 2 (PTGR2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTGR2. No significant cross-reactivity or interference between Human PTGR2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PTGR2 is an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. ZADH1 has a domain characteristic of the zinc-containing ADH family , a proline-rich motif, and a putative NADP(+)-binding domain. RT-PCR detected ubiquitous expression of the shorter ZADH1 transcript (ZADH1b), with highest expression in kidney, liver, pancreas, prostate, and heart, followed by lung, skeletal muscle, testis, small intestine, and brain. Expression was low in all other tissues examined.
Gene ID 145482
Alternative PTGR2; DKFZp686P10120; FLJ39091; FLJ99229; PGR2; ZADH1; 15-oxoprostaglandin-delta13-reductase; zinc binding alcohol dehydrogenase; domain containing 1
Accession Q8N8N7

Image & description

Fig.1. Human Prostaglandin reductase 2 (PTGR2) Standard Curve.

Fig.1. Human Prostaglandin reductase 2 (PTGR2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Prostaglandin reductase 2 (PTGR2) ELISA Kit”