- Product name
IFKine™ Red Donkey Anti-Rabbit IgG
Rabbit IgG whole molecule
FCM, ICC, IF
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE
- Features & Benefits
Abbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background). The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling).
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.
The antibody reacts with whole molecule rabbit IgG. It also reacts with light chains of all other rabbit immunoglobulins. It's cross-reaction with human, mouse, rat, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins
Most popular with customers
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Application: ELISA, IHC, WB
Application: ELISA, IHC, WB
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Spinal CX3CL1/CX3CR1 May Not Directly Participate in the Development of Morphine Tolerance in Rats
Peng Y, Guo G, Shu B, et al. Neurochem Res, 2017, 42(11): 3254-3267.
Neuropilin-1 promotes primary liver cancer progression by potentiating the activity of hepatic stellate cells
Xu Z C, Shen H X, Chen C, et al. Oncol Lett, 2018, 15(2): 2245-2251.
EV71 3D Protein Binds with NLRP3 and Enhances the Assembly of Inflammasome Complex
Wang, Wenbiao, et al. PLoS pathogens 13.1 (2017): e1006123.
Thioredoxin-interacting protein links endoplasmic reticulum stress to inflammatory brain injury and apoptosis after subarachnoid haemorrhage
Zhao, Qing, et al. Journal of neuroinflammation 14.1 (2017): 104.
Berberine improves motor function recovery byinhibiting endoplasmic reticulum stress-inducedneuronal apoptosis via AMPK activation in ratswith spinal cord injury
Wang, Jiquan, et al. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.4 (2017): 4900-4911.
Protective effects of hydrogen sulfide on acute spinal cord injury in rats
Sun Ping, et al. Tianjin Medical Journal, 43.11 (2015): 1271-1274.
Expression of p96 in the inner ear hair cells in mice
Chen,Zhiji,et al. Chinese Journal of Ophthalmology and Otorhinolaryngology, 15.5(2015): 315-317.
Research on the interaction of protein Tmub1 and securin after partial hepatectomy
ZHAO, Xiao-biao, et al. Medical Journal of Chinese People's Liberation Army 39.11 (2014): 864-867.
Leptin/leptinR-kisspeptin/kiss1r-GnRH pathway reacting to regulate puberty onset during negative energy balance.
Luo Q, Li W, Li M, et al. Life sciences, 2016, 153: 207-212.
Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice.
Chen H, Sun Y, Lai L, et al. Neuroscience, 2015, 308: 157-168.
Membrane receptor-dependent Notch1/Hes1 activation by melatonin protects against myocardial ischemia–reperfusion injury: in vivo and in vitro studies.
Yu L, Liang H, Lu Z, et al. Journal of pineal research, 2015, 59(4): 420-433.
Exendin-4 Enhances Motor Function Recovery via Promotion of Autophagy and Inhibition of Neuronal Apoptosis After Spinal Cord Injury in Rats.
Li H T, Zhao X Z, Zhang X R, et al. Molecular neurobiology, 2015: 1-10.
Mitochondrial Division Inhibitor 1 Ameliorates Mitochondrial Injury, Apoptosis, and Motor Dysfunction After Acute Spinal Cord Injury in Rats.
Li G, Jia Z, Cao Y, et al. Neurochemical research, 2015, 40(7): 1379-1392.
FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells.
Zhang L, Wang H, Ding K, et al. Toxicology letters, 2015, 236(1): 43-59.
Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus.
Su X, Yan H, Huang Y, et al. Scientific reports, 2015, 5.