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Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit

Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit

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Specification

Product name Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2 in samples. An antibody specific for P4HA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative P4HA2; 4-PH alpha 2; C-P4Halpha(II); OTTHUMP00000065966; OTTHUMP00000065969; collagen prolyl 4-hydroxylase alpha(II); procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypepti

Product Properties

Kit components • Human Prolyl 4-hydroxylase subunit alpha-2 microplate
• Human Prolyl 4-hydroxylase subunit alpha-2 standard
• Human Prolyl 4-hydroxylase subunit alpha-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human P4HA2. No significant cross-reactivity or interference between Human P4HA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. The beta subunit (P4HB) is an unusual multifunctional polypeptide identical to protein disulfide isomerase. P4HA2 is one of at least 2 alpha subunit isoforms. This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains.
Gene ID 8974
Alternative P4HA2; 4-PH alpha 2; C-P4Halpha(II); OTTHUMP00000065966; OTTHUMP00000065969; collagen prolyl 4-hydroxylase alpha(II); procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypepti
Accession O15460

Image & description

Fig.1. Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) Standard Curve.

Fig.1. Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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