| Product name | EliKine™ Human TSH ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human TSH ELISA Kit employs a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for TSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person. |
| Alternative | Thyrotropin subunit beta; TSH A; TSH alpha; TSH B; TSH beta; TSHA; TSHB; Thyroid stimulating hormone; Thyroid stimulating hormone alpha chain |
| Kit components | • Human TSH microplate • Human TSH standard • Human TSH detect antibody • HRP substrate A • HRP substrate B • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Human TSH ELISA Kit has high sensitivity and excellent specificity for detection of Human TSH. No significant cross-reactivity or interference between Human TSH and analogues was observed. |
| Calibration range | 0.3 mIU/L-12 mIU/L |
| Limit of detection | 0.1 mIU/L |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for |
| Storage instructions | The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone, TSH, or hTSH for human TSH) is a pituitary hormone that stimulates the thyroid gland to produce thyroxine (T4), and then triiodothyronine (T3) which stimulates the metabolism of almost every tissue in the body. It is a glycoprotein hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid. In 1916, Bennett M. Allen and Philip E. Smith found that the pituitary contained a thyrotropic substance. |
| Gene ID | 1081 |
| Alternative | Thyrotropin subunit beta; TSH A; TSH alpha; TSH B; TSH beta; TSHA; TSHB; Thyroid stimulating hormone; Thyroid stimulating hormone alpha chain |
| Accession | P01215 |
Fig.1. Human TSH Standard Curve.
Fig.2. EliKine™ Human TSH ELISA Kit is a sandwich ELISA to quantitate TSH in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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