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Mouse Tight junction protein ZO-2 (TJP2) ELISA Kit

Mouse Tight junction protein ZO-2 (TJP2) ELISA Kit

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Specification

Product name Mouse Tight junction protein ZO-2 (TJP2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Tight junction protein ZO-2 (TJP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TJP2 in samples. An antibody specific for TJP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTJP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TJP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TJP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TJP2; RP11-16N10.1; MGC26306; X104; ZO2; Friedreich ataxia region gene X104 (tight junction protein ZO-2); tight junction protein 2; tight junction protein ZO 2; zonula occludens protein 2

Product Properties

Kit components • Mouse Tight junction protein ZO-2 microplate
• Mouse Tight junction protein ZO-2 standard
• Mouse Tight junction protein ZO-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Tight junction protein ZO-2 (TJP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TJP2. No significant cross-reactivity or interference between Mouse TJP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TJP2 encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutation in this gene have been identified in patients with hypercholanemia. Alternate splicing results in multiple transcript variants. This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain whereas isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. In brain and skeletal muscle, only isoform A1 is detectable. Isoform C1 is found in normal as well as in most neoplastic tissues while isoform A1 is present almost exclusively in normal tissue.
Gene ID 21873
Alternative TJP2; RP11-16N10.1; MGC26306; X104; ZO2; Friedreich ataxia region gene X104 (tight junction protein ZO-2); tight junction protein 2; tight junction protein ZO 2; zonula occludens protein 2
Accession Q9Z0U1

Image & description

Fig.1. Mouse Tight junction protein ZO-2 (TJP2) Standard Curve.

Fig.1. Mouse Tight junction protein ZO-2 (TJP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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