Product Information
- Product name
Human Procollagen III C-terminal Peptide (PIIICP) ELISA Kit
- Reactivity
Human
- Applications
ELISA
- Application notes
This Human Procollagen III C-terminal Peptide (PIIICP) ELISA Kit employs a two-site sandwich ELISA to quantitate PIIINP in samples. An antibody specific for PIIINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIIINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIIINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIIINP bound in the initial step. The color development is stopped and the intensity of the color is measured.
- Detection method
Colorimetric
- Sample type
Cell culture supernatants, Other biological fluids, Plasma, Serum
- Assay type
Sandwich ELISA (quantitative)
- Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Product Properties
- Kit components
• Human Procollagen III C-terminal Peptide microplate
• Human Procollagen III C-terminal Peptide standard
• Human Procollagen III C-terminal Peptide detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers - Features & Benefits
Human Procollagen III C-terminal Peptide (PIIICP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIIINP. No significant cross-reactivity or interference between Human PIIINP and analogues was observed.
- Calibration range
Please inquire
- Limit of detection
Please inquire
- Usage notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate. - Storage instructions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
- Shipping
Gel pack with blue ice.
- Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Additional Information
- Background
PIIICP occurs as a trimer consisting of three identical monomeric PIIICP subunits that are linked by intermolecular disulfide bridges. Structural considerations and site-directed mutagenesis experiments with a collagen minigene have led to the conclusion that at least 4 and probably 6 cysteine residues are involved in intramolecular disulfide bridge formation and that only cysteines 51 and 68 are involved in intermolecular disulfide bridge formation. It has been observed, however, that the region around this intermolecular cystine bridge is critical for the correct formation of intramolecular disulfide bridges and that a Cys->Ser mutation in that region also leads to impaired intramolecular disulfide bridge formation. The PIIICP cDNA sequence has been published and two entries are found in the Genbank data base. The sequence was identical to the sequence published by Ala-Kokko et al., 1989, and deviated from the sequence published by Loidl et al. (1984) in one amino acid.
- Alternative names
PIIINP
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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.
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