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IFKine™ Green Donkey Anti-Rabbit IgG

IFKine™ Green conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 518 nm. They are brighter than FITC, Cy2, and DyLight 488. IFKine™ Green conjugates are recommended for for all immunofluorescence procedures requiring a green-fluorescing dye.

Product Information

  • Product name

    IFKine™ Green Donkey Anti-Rabbit IgG

  • Immunogen

    Rabbit IgG whole molecule

  • Host


  • Reactivity


  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

  • Clonality


  • Isotype

    Donkey IgG

  • Purification

    Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    Abbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.

  • Others

    The antibody reacts with whole molecule rabbit IgG. It also reacts with light chains of all other rabbit immunoglobulins. It's cross-reaction with human, mouse, rat, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Overexpression Of miR138 Ameliorates Spared Sciatic Nerve Injury-Induced Neuropathic Pain Through The Anti-Inflammatory Response In Mice

    B Zhu, J Gao, Y Ouyang. Journal of Pain Research

  2. Electroacupuncture at ST-36 ameliorates DSS-induced acute colitis via regulating macrophage polarization induced by suppressing NLRP3/IL-1β and promoting Nrf2/HO-1

    Shuangning Song, Jing An, Yingli Li. Molecular Immunology.

  3. Electroacupuncture at ST-36 ameliorates DSS-induced acute colitis via regulating macrophage polarization induced by suppressing NLRP3/IL-1β and promoting Nrf2.

    S Song, J An, Y Li, S Liu - Molecular immunology, 2019.

  4. Ventrolateral Periaqueductal Gray Matter Neurochemical Lesion Facilitates Epileptogenesis and Enhances Pain Sensitivity in Epileptic Rats

    Lei Wang, Jie Shen, Xin-Ting Cai, et al. Neuroscience. 2019 Jul 15;411:105-118.

  5. Triptolide exerts an anti-tumor effect on non‑small cell lung cancer cells by inhibiting activation of the IL‑6/STAT3 axis

    Ying Huang, Zhe Chen, Yu Wang, et al. Int J Mol Med. 2019 Jul;44(1):291-300.

  6. miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A

    Xiangdong Liu, Wenzhong Zhu, Lei Wang, et al. In Vitro Cell Dev Biol Anim. 2019 Mar;55(3):189-202.

  7. TRPV4 inhibition improved myelination and reduced glia reactivity and inflammation in a cuprizone-induced mouse model of demyelination

    Meiying Liu, Xuan Liu, et al. Frontiers Cell Neuroscience. 2018; 12: 392.

  8. Pro-resolving mediator maresin 1 ameliorates pain hypersensitivity in a rat spinal nerve ligation model of neuropathic pain

    Jie Gao, Chaoliang Tang, et al. Journal of Pain Research. 2018; 11: 1511-1519.

  9. Acute Hypoxia Induced an Imbalanced M1/M3 Activation of Microglia through NF-κB Signaling in Alzheimer's Disease Mice and Wild-Type Littermates

    Zhang F, Zhong R, Li S, et al. Front Aging Neurosci, 2017, 25; 9: 283.

  10. Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma

    Zeng, Guangping, et al. Oncotarget 8.3 (2017): 4607.

  11. The pro-resolving lipid mediator Maresin 1 protects against cerebral ischemia/reperfusion injury by attenuating the pro-inflammatory response.

    Xian W, Wu Y, Xiong W, et al. Biochemical and biophysical research communications, 2016, 472(1): 175-181.

  12. Classically Activated Macrophages Protect against Lipopolysaccharide-induced Acute Lung Injury by Expressing Amphiregulin in Mice.

    Xu Y, Meng C, Liu G, et al. The Journal of the American Society of Anesthesiologists, 2016, 124(5): 1086-1099.

Cat #: A24221

  • Size
  • Price
  • 100μl $70
  • 500μl $280

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer:
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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