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Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) ELISA Kit

Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) ELISA Kit

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Specification

Product name Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) ELISA Kit employs a two-site sandwich ELISA to quantitate KCNA2 in samples. An antibody specific for KCNA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KCNA2; HBK5; HK4; HUKIV; KV1.2; MGC50217; MK2; NGK1; RBK2; potassium channel; voltage-gated potassium channel protein Kv1.2

Product Properties

Kit components • Human Potassium voltage-gated channel subfamily A member 2 microplate
• Human Potassium voltage-gated channel subfamily A member 2 standard
• Human Potassium voltage-gated channel subfamily A member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human KCNA2. No significant cross-reactivity or interference between Human KCNA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). KCNA2 encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of KCNA2 is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1.
Gene ID 3737
Alternative KCNA2; HBK5; HK4; HUKIV; KV1.2; MGC50217; MK2; NGK1; RBK2; potassium channel; voltage-gated potassium channel protein Kv1.2
Accession P16389

Image & description

Fig.1. Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) Standard Curve.

Fig.1. Human Potassium voltage-gated channel subfamily A member 2 (KCNA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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