- Product name
HRP, Goat Anti-Rabbit IgG
Rabbit IgG whole molecule
ELISA, IHC, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10,000-1:100,000), IHC (1:500-1:2,000) and ELISA (1:5,000-1:100,000).
Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE.
- Storage buffer
Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
Most popular with customers
Application: FCM, ICC, IF
Application: IP, WB
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.
The deubiquitinating enzyme cylindromatosis mitigates nonalcoholic steatohepatitis
Ji YX, Huang Z, Yang X et al. Nat Med. 2018, 24(2): 213-223.
MicroRNA-155-3p Mediates TNF-α-Inhibited Cementoblast Differentiation
Wang X, Sun H, Liao H. J Dent Res.2017, 96(12): 1430-1437.
miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat
Ling YH, Sui MH, Zheng Q, et al. Sci Rep, 2018, 8(1): 3909.
The miR-486-5p plays a causative role in prostate cancer through negative regulation of multiple tumor suppressor pathways
Yang Y, Ji C, Guo S, et al. Oncotarget, 2017, 8(42): 72835-72846.
Nanoparticle-Shaped Titanium Promotes Bone Mesenchymal Stem Cell Adhesion Through Integrin α5β2
Sun F F, Mao P, Lu X L, et al. Nanoscience and Nanotechnology Letters, 2017, 9: 11.
Persistent STAT5-mediated ROS production and involvement of aberrant p53 apoptotic signaling in the resistance of chronic myeloid leukemia to imatinib
Cheng Y, Hao Y, Zhang A, et al. Int J Mol Med, 2018, 41(1): 455-464.
Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibitingproliferation and inducing death of human non-small cell lung cancer cells
Zheng X, Xu W, Sun R, et al. Chem Biol Interact, 2017, 25; 275: 74-85.
Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata
Zhang H, Huang X, Shi Y, et al. Fish Shellfish Immunol, 2018, 73: 279-287.
MicroRNA-21 antisense oligonucleotide improves the sensitivity of A375 human melanoma cell to Cisplatin: An in vitro study
Zhang H L, Si L B, Zeng A, et al. J Cell Biochem, 2018, 119(4): 3129-3141.
A New Immunosuppressive Molecule Emodin Induces both CD4+FoxP3+ and CD8+CD122+ Regulatory T Cells and Suppresses Murine Allograft Rejection
Qiu F, Liu H, Liang C L, et al. Front Immunol, 2017, 8; 8: 1519.
Ethyl acetate extract from Inula helenium L inhibits the proliferation of pancreatic cancer cells by regulating the STAT3/AKT pathway
Zhang B, Zeng J, Yan Y, et al. 2018, 17(4): 5440-5448.
High-glucose induces tau hyperphosphorylation through activation of TLR9-P39MAPK pathway
Sun Y, Xiao Q, Luo C, et al. Exp Cell Res, 2017, 15; 359(2): 312-319.
FK506 suppresses hypoxia‑induced inflammation and protects tight junction function via the CaN‑NFATc2 signaling pathway in retinal microvascular epithelial cells
Hu Y, Yang B, Xu Y, et al. Mol Med Rep, 2017, 16(5): 6974-6981.
miR-434 accelerates acquired chemoresistance of gallbladder cancer cells by targeting cyclin M
Yu J, Zhang W, Lu B, et al. Oncol Lett, 2018, 15(3): 3305-3313.
Overexpression of Bmi‑1 promotes epithelial‑mesenchymal transition in CD133+Hep G2 cells
Zhang Z, Wang Q, Bu X, et al. Mol Med Rep, 2017, 16(5): 6156-6162.
Osthole inhibits the PI4K/AKT signaling pathway via activation of PTEN and induces cell cycle arrest and apoptosis in esophageal squamous cell carcinoma
Zhu X, Li Z, Li T, et al. Biomed Pharmacother, 2018, 23; 102: 502-510.
lgtF effects of Haemophilus parasuis LOS induced inflammation through regulation of NF-κB and MAPKs signaling pathways
Zeng Z, Zhang B, He H, et al. Microb Pathog, 2017, 110: 380-385.
Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E4 ubiquitin ligase
Liu X J, Yang B, Huang SN, et al. PLoS Pathog, 2017, 13(7): e1006543.
Protein self-assembly via Zr4+ ions on spore-based microspheres for immunoassays
Xia M M, Li Z, Chen Y Y, et al. Sensors and Actuators B: Chemical, 2018, 254: 166-176.
KDM5A promotes proliferation and EMT in ovarian cancer and closely correlates with PTX resistance
Feng T, Wang Y, Lang Y, et al. Mol Med Rep, 2017, 16(3): 3573-3580.
Nuclear factor 90 promotes angiogenesis by regulating HIF-1α/VEGF-A expression through the PI3K/Akt signaling pathway in human cervical cancer
Zhang W, Xiong Z, Wei T, et al. Cell Death Dis, 2018, 9(3): 276.
Paired related homeobox protein 1 regulates PDGF-induced chemotaxis of hepatic stellate cells in liver fibrosis
Gong J, Han J, He J, et al. Lab Invest, 97(9): 1020-1032.
Long non‑coding RNA MEG3 contributes to cisplatin‑induced apoptosis via inhibition of autophagy in human glioma cells
Ma B B, Gao Z B, Lou J C, et al. Molecular Medicine Reports, 2017, 16(3): 2946-2952.
Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
Dong Z, Chen J, Yang X, et al. Oncotarget, 2018.
Cavin-1 regulates caveolae-mediated LDL transcytosis: crosstalk in an AMPK/eNOS/NF-κB/Sp1 loop
Bai X L, Yang X Y, Li J Y, et al. Oncotarget, 2018.
The dual role of poly(ADP-ribose) polymerase-1 in modulating parthanatos and autophagy under oxidative stress in rat cochlear marginal cells of the stria vascularis
Jiang H Y, Yang Y, Zhang Y Y, et al. Redox Biology, 2018, 14: 361-370.
Tmbim1 is a multivesicular body regulator that protects against non-alcoholic fatty liver disease in mice and monkeys by targeting the lysosomal degradation of Tlr4
Zhao, Guang-Nian, et al. Nature Medicine 23.6 (2017): 742-752.
TNF-α stimulates endothelial palmitic acid transcytosis and promotes insulin resistance
Li, Wenjing, et al. Scientific Reports 7 (2017).
Qiliqiangxin protects against anoxic injury in cardiac microvascular endothelial cells via NRG-1/ErbB-PI3K/Akt/mTOR pathway
Wang, Jingfeng, et al. Journal of Cellular and Molecular Medicine (2017).
Silymarin inhibits adipogenesis in the adipocytes in grass carp Ctenopharyngodon idellus in vitro and in vivo
Xiao, Peizhen, et al. Fish Physiology and Biochemistry (2017): 1-14.
AG490 suppresses interleukin-34-mediated osteoclastogenesis in mice bone marrow macrophages
Cheng, Xin, Qi‐Long Wan, and Zu‐Bing Li. Cell Biology International 41.6 (2017): 659-668.
Maintenance of the contractile phenotype in corpus cavernosum smooth muscle cells by Myocardin gene therapy ameliorates erectile dysfunction in bilateral cavernous nerve injury rats
Zhang, H.B., et al. Andrology (2017).
Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats
Wang, Jingfeng, et al. Evidence-Based Complementary and Alternative Medicine 2017 (2017).
CDKL1 promotes tumor proliferation and invasion in colorectal cancer
Qin, Chunzhi, et al. OncoTargets and therapy 10 (2017): 1613.
LAMC1 mRNA promotes malignancy of hepatocellular carcinoma cells by competing for MicroRNA-124 binding with CD151
Yang, Zhan‐Po, et al. IUBMB life (2017).
IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes
Wang, Bing, et al. Mediators of Inflammation 2017 (2017).
MicroRNA-384-mediated Herpud1 upregulation promotes angiotensin II-induced endothelial cell apoptosis
Lin, Hui, et al. Biochemical and Biophysical Research Communications 488.3 (2017): 453-460.
Effects of Total Flavone from Rhododendron simsii Planch. Flower on Postischemic Cardiac Dysfunction and Cardiac Remodeling in Rats
Cheng, Xinqi, et al. Evidence-Based Complementary and Alternative Medicine 2017 (2017).
Hydrogen coadministration slows the development of COPD-like lung disease in a cigarette smoke-induced rat model
Liu, Xiaoyu, et al. International journal of chronic obstructive pulmonary disease 12 (2017): 1309.
Sodium Salicylate Modulates Inflammatory Responses Through AMP-Activated Protein Kinase Activation in LPS-stimulated THP-1 Cells
Bao, Weiwei, et al. Journal of Cellular Biochemistry (2017).
SIRT6 inhibits TNF-α-induced inflammation of vascular adventitial fibroblasts through ROS and Akt signaling pathway
He, Yanhao, et al. Experimental Cell Research (2017).
Runx2 alleviates high glucose-suppressed osteogenic differentiation via PI3K/AKT/GSK3β/β-catenin pathway
Chen, Yang, et al. Cell Biology International (2017).
The Role of Spinal GABAB Receptors in Cancer-Induced Bone Pain in Rats
Zhou, Ya-Qun, et al. The Journal of Pain (2017).
Effect of TGF-β1 on the expression of collagen in rat atrial and ventricular fibroblasts
LIU, Fa-jin, et al. Medical Journal of Chinese People's Liberation Army 40.7 (2015): 540-546.
Early growth response 3 inhibits growth of hepatocellular carcinoma cells via upregulation of Fas ligand
Zhang, S, et al. International Journal of Oncology (2017).
Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen β-Conglycinin
Wang, Yao, et al. Food Analytical Methods (2017): 1-7.
Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway
Zheng, T, et al. Journal of Ethnopharmacology 193(2016): 691-699
A hybrid siRNA delivery complex for enhanced brain penetration and precise amyloid plaque targeting in Alzheimer's disease mice
Zheng, Xiaoyao, et al. Acta Biomaterialia (2016).
OSM mitigates post-infarction cardiac remodeling and dysfunction by up-regulating autophagy through Mst1 suppression
Hu, Jianqiang, et al. Biochim Biophys Acta (2016).
A Novel Mechanism of Action for Salidroside to Alleviate Diabetic Albuminuria: Effects on Albumin Transcytosis across Glomerular Endothelial Cells
Wu, D, et al. American Journal of Physiology-Endocrinology and Metabolism, 2016, 310(3): E225-E237.
Suppression of endothelial PGC-1α is associated with hypoxia-induced endothelial dysfunction and provides a new therapeutic target in pulmonary arterial hypertension
Ye, Jia-Xin, et al. American Journal of Physiology-Lung Cellular and Molecular Physiology 310.11 (2016): L1233-L1242.
NPAS3 regulates transcription and expression of VGF: implications for neurogenesis and psychiatric disorders
Yang, Dongxue, et al. Frontiers in Molecular Neuroscience 9 (2016).
Study on the Mutual Induction between Tooth Germ Cells and Bone Marrow Mesenchymal Stem Cells Transfected by Bone Morphogenetic Protein4 Gene in Vitro
Wang, Huanhuan, et al. Journal of ORAL Science Research, 31.3 (2015): 237-241.
Effect and mechanism of acidification on vascular calcification in chronic renal failure rats
Zhang Shenglei, et al. Chinese Journal of Nephrology,31.12 (2015): 924-931.
Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.
Liu H, Li M, Cai S, et al. Acta Biochimica et Biophysica Sinica, 2016.
Bmi-1 promotes the invasion and migration of colon cancer stem cells through the downregulation of E-cadherin.
Zhang Z, Bu X, Chen H, et al. International Journal of Molecular Medicine, 2016, 38: 1199-1207.
Ficolin-2 binds to HIV-1 gp120 and blocks viral infection.
Luo F, Chen T, Liu J, et al. Virologica Sinica, 2016: 1-9.
The ubiquitin E3 ligase TRAF6 exacerbates pathological cardiac hypertrophy via TAK1-dependent signalling.
Ji Y X, Zhang P, Zhang X J, et al. Nature communications, 2016, 7.
Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells.
Du R, Liu Z, Hou X, et al. Molecular medicine reports, 2016, 13(6): 5045-5052.
Blocking mPTP on Neural Stem Cells and Activating the Nicotinic Acetylcholine Receptor α7 Subunit on Microglia Attenuate Aβ-Induced Neurotoxicity on Neural Stem Cells.
Chen Q, Wang K, Jiang D, et al. Neurochemical research, 2016, 41(6): 1483-1495.
Electroacupuncture relieves labour pain and influences the spinal dynorphin/κ-opioid receptor system in rats.
Jiang Q Y, Wang M Y, Li L, et al. Acupuncture in Medicine, 2016, 34(3): 223-228.
Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.
Gao L, Xu Z, Wang Y, et al. Oncology reports, 2016, 35(2): 1101-1108.
IL-17 intensifies IFN-γ-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-κB.
Gao Q, Liu Y, Wu Y, et al. International journal of molecular medicine, 2016, 37(2): 347-358.
The Protein Elicitor PevD1 Enhances Resistance to Pathogens and Promotes Growth in Arabidopsis.
Liu M, Khan N U, Wang N, et al. International Journal of Biological Sciences, 2016, 12(8): 931.
Restoration of murine femoral segmental defect using CTGF-overexpressing MC3T3-E1 cells.
Huang X, Li Y, Xu J, et al. American journal of translational research, 2016, 8(3): 1530.
PfIRR Interacts with HrIGF-I and Activates the MAP-kinase and PI3-kinase Signaling Pathways to Regulate Glycogen Metabolism in Pinctada fucata.
Shi Y, He M. Scientific reports, 2016, 6.
The regulation of glycogen synthase kinase-3β on the mesenchymal stem cell therapy in the lypopolysaccharide induced acute lung injury in rat.
Lkhamsuren B, Wang H, Gao P, et al. Int J Clin Exp Med, 2016, 9(4): 7102-7114.
Salidroside improves endothelial function and alleviates atherosclerosis by activating a mitochondria-related AMPK/PI3K/Akt/eNOS pathway.
Xing S S, Yang X Y, Zheng T, et al. Vascular pharmacology, 2015, 72: 141-152.
Curcumin induces M2 macrophage polarization by secretion IL-4 and/or IL-13.
Gao S, Zhou J, Liu N, et al. Journal of molecular and cellular cardiology, 2015, 85: 131-139.
Salidroside ameliorates insulin resistance through activation of a mitochondria-associated AMPK/PI3K/Akt/GSK3β pathway.
Zheng T, Yang X, Wu D, et al. British journal of pharmacology, 2015, 172(13): 3284-3301.
Rapid and sensitive detection of the food allergen glycinin in powdered milk using a lateral flow colloidal gold immunoassay strip test.
Wang Y, Deng R, Zhang G, et al. Journal of agricultural and food chemistry, 2015, 63(8): 2172-2178.
TLR4 inhibitor resatorvid provides neuroprotection in experimental traumatic brain injury: implication in the treatment of human brain injury.
Zhang D, Li H, Li T, et al. Neurochemistry international, 2014, 75: 11-18.