|Product name||Human Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit|
|Applications notes||This Human Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGF-BB in samples. An antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGF-BB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-BB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Platelet-Derived Growth Factor-BB microplate
• Human Platelet-Derived Growth Factor-BB standard
• Human Platelet-Derived Growth Factor-BB detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGF-BB. No significant cross-reactivity or interference between Human PDGF-BB and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The Platelet-Derived Growth Factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, -DD)and one heterodimer (PDGF-AB). These proteins and the related VEGF family proteinsshare the conserved PDGF/VEGF homology domain characterized by a pattern of highly conserved cysteine residues, which form the cysteine knot motif. PDGF-B is synthesized as a pre-pro-protein, which has a signal peptide, a mature region, an N- terminal pro-peptide, and a C-terminal extension. The C-terminal extension contains a conserved cell retention motif that confers retention of the secreted PDGF-BB within the pericellular space. Most cells produce both PDGF-A and B chains. The individual chains are assembled stochastically into disulfide-linked inactive homodimeric or heterodimeric precursors in the endoplasmic reticulum.|
Fig.1. Human Platelet-Derived Growth Factor-BB (PDGF-BB) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.