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Human USP6 N-terminal-like protein (USP6NL) ELISA Kit

Human USP6 N-terminal-like protein (USP6NL) ELISA Kit

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Specification

Product name Human USP6 N-terminal-like protein (USP6NL) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human USP6 N-terminal-like protein (USP6NL) ELISA Kit employs a two-site sandwich ELISA to quantitate USP6NL in samples. An antibody specific for USP6NL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP6NL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP6NL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP6NL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative USP6NL; KIAA0019; RNTRE; TRE2NL; related to the N terminus of tre

Product Properties

Kit components • Human USP6 N-terminal-like protein microplate
• Human USP6 N-terminal-like protein standard
• Human USP6 N-terminal-like protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human USP6 N-terminal-like protein (USP6NL) ELISA Kit has high sensitivity and excellent specificity for detection of Human USP6NL. No significant cross-reactivity or interference between Human USP6NL and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HAUSPis a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress.
Gene ID 9712
Alternative USP6NL; KIAA0019; RNTRE; TRE2NL; related to the N terminus of tre
Accession Q92738

Image & description

Fig.1. Human USP6 N-terminal-like protein (USP6NL) Standard Curve.

Fig.1. Human USP6 N-terminal-like protein (USP6NL) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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