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Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit

Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit

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Specification

Product name Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit employs a two-site sandwich ELISA to quantitate PAM in samples. An antibody specific for PAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PAM; PAL; PHM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylglycine 2-hydroxylase; peptidylglycine a

Product Properties

Kit components • Human Peptidyl-glycine alpha-amidating monooxygenase microplate
• Human Peptidyl-glycine alpha-amidating monooxygenase standard
• Human Peptidyl-glycine alpha-amidating monooxygenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAM. No significant cross-reactivity or interference between Human PAM and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Peptidyl-glycine alpha-amidating monooxygenase is a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known.
Gene ID 5066
Alternative PAM; PAL; PHM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylglycine 2-hydroxylase; peptidylglycine a
Accession P19021

Image & description

Fig.1. Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) Standard Curve.

Fig.1. Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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