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Human Pannexin-1 (PANX1) ELISA Kit

Human Pannexin-1 (PANX1) ELISA Kit

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Specification

Product name Human Pannexin-1 (PANX1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Pannexin-1 (PANX1) ELISA Kit employs a two-site sandwich ELISA to quantitate PANX1 in samples. An antibody specific for PANX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPANX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PANX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PANX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PANX1; MGC21309; MRS1; PX1; UNQ2529; innexin

Product Properties

Kit components • Human Pannexin-1 microplate
• Human Pannexin-1 standard
• Human Pannexin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Pannexin-1 (PANX1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PANX1. No significant cross-reactivity or interference between Human PANX1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pannexin 1 belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties. The deduced 48-kD protein contains 4 transmembrane domains and cytoplasmic N and C termini. Northern blot analysis detected Panx1 expression in rat eye, thyroid, prostate, kidney, liver, and central nervous system. In situ hybridization showed Pnx1 expression in several brain regions, including cortex, striatum, olfactory bulb, hippocampus, thalamus, and cerebellum.
Gene ID 24145
Alternative PANX1; MGC21309; MRS1; PX1; UNQ2529; innexin
Accession Q96RD7

Image & description

Fig.1. Human Pannexin-1 (PANX1) Standard Curve.

Fig.1. Human Pannexin-1 (PANX1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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