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Human Osteopontin (OPN) ELISA Kit

Human Osteopontin (OPN) ELISA Kit

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Specification

Product name Human Osteopontin (OPN) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Osteopontin (OPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPP1 in samples. An antibody specific for SPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPP1; BNSP; BSPI; ETA-1; MGC110940; OPN; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; s

Product Properties

Kit components • Human Osteopontin microplate
• Human Osteopontin standard
• Human Osteopontin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Osteopontin (OPN) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPP1. No significant cross-reactivity or interference between Human SPP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Osteopontin (OPN), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling , cell survival, and tumor progression . Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes BSP,BMP1,DSPP, ENAM and MEPE. Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN.
Gene ID 6696
Alternative SPP1; BNSP; BSPI; ETA-1; MGC110940; OPN; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; s
Accession P10451

Image & description

Fig.1. Human Osteopontin (OPN) Standard Curve.

Fig.1. Human Osteopontin (OPN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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